AIM - In vitro clonal propagation of Acacia nilotica (L.) - A nitrogen fixing tree
| A. nilotica
Acacia nilotica (L.) wild ex Del belongs to the family Leguminosae commonly known as Babool or Kikar, is a multipurpose legume tree. Acacia nilotica (gum Arabic tree, babul, Egyptian thorn, Sant tree, Al-sant or prickly acacia; called thorn mimosa in Australia; lekkerruikpeul or scented thorn in South Africa) is a species of Acacia (wattle) native to Africa and the Indian subcontinent. It acts as a biological control agent for herbivores. It is found in the central belt of Indian subcontinent and Australia. This plant has been used as a source of pulpwood, timber, fodder and gum. The whole parts of the plant used for medicinal purposes like barks act as astringent which found to be useful in the treatment of dysentery, diarrhea, leprosy and smallpox. This plant also shows antioxidant activity. The leguminous trees are one of the most significant components of forest vegetation due to their economic and ecological importance. However, the regeneration rate of this plant in natural surroundings is quite low. In general, the woody plants are difficult to regenerate under in vitro conditions but some success was achieved in a few leguminous tree species except Acacia nilotica. In vitro regeneration protocols have been standardized earlier in some other species of Acacia like A. mearnsii De Wild, A. catechu Willd, A. sinuate, A. chundra, A. Senegal except A. nilotica(L.). However, a large number of publications are available regarding its antimicrobial activity, antioxidant activity. This experiment describes a successful protocol on in vitro propagation of A. nilotica from nodal explants of in vitro grown plants. An efficient regeneration protocol was developed for in vitro propagation of Acacia nilotica (L.), a nitrogen fixing tree, through direct regeneration. In vitro nodal segments cultured on Murashige and Skoog (MS) medium supplemented with NAA (0.6 mg/l) and Kinetin (1.0 mg/l) for shoot proliferation. NAA was found to be more effective than Kinetin for shoot multiplication. The highest number of shoots (4.6±0.7) was achieved on MS medium augmented with NAA (0.6 mg/l). However, excised shoots (2-3cm) were rooted on half strength MS medium supplemented with IBA (0.5 mg/l) after 15-20 days of culture. The micropropagated plantlets were hardened and acclimatized. They were successfully transferred to natural conditions with 75% survival rate.
MATERIALS AND METHODS
MATERIALS - Kinetin (0, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0 mg/l), 1-naphthaleneacetic acid (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg/l), Indole-3-butyric acid (0.1,0.3,0.5,0.7 mg/l), IAA (2 mg/l and 3 mg/l), BAP (1.5 mg/l) and BAP (1mg/l), Seeds of Acacia nilotica L, 1% (v/v) Teepol, double distilled water, 0.1% (w/v) aqueous HgCl2 , Laminar Air flow cabinet, MS salts medium(3 % sucrose, 3 % sucrose, kinetin, NAA, IBA), filter paper bridges, filter paper disk and on non-absorbent cotton, Petri plates, polycups with vermicompost
I. Seed germination and procurement of explants
1. Seeds of Acacia nilotica L. were collected.
2. Prior to surface sterilization, seeds were soaked in distilled water for about 48 hrs. 3. Then they are kept under running tap water for about 10-15 mins. 4. Followed by washing with 1% (v/v) Teepol for 2 min.
5. The seeds are rinsed with double distilled water for three times. 6. Prior to inoculation, seeds were further sterilized with 0.1% (w/v) aqueous HgCl2 for about 2 mins. 7. Rinsed 2-3 times with double distilled water in Laminar Air flow cabinet. 8. These sterilized seeds were inoculated on half and full strength MS salts medium, filter paper bridges, filter paper disk and on non-absorbent cotton in the Petri...
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